Preclinical Research Services

Our interdisciplinary team has a wide range of skills and expertise in in vivo preclinical investigations to de-risk products, utilizing various species (mouse, rat and rabbit) for proof-of-concept studies and non-GLP IND-enabling studies.

Ocular Disease Models, Proof of Concept, and Efficacy

C20/20 offers in vivo models across several species mimicking human disease states to validate a novel therapeutic approach. We help you select the appropriate in vivo model for your needs and through consultation are also able to generate new animal models or surgical approaches as needed, tailored to your specific needs. We have state-of-the-art equipment including optical coherence tomography (OCT), electroretinographs (ERG), a variety of fundus imaging modalities, and much more to generate both morphological and functional data to fully characterize model rescue.

Below are some specific applications of various models the C20/20 team can accommodate:

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Angiogenesis & Permeability Models

Applications:

  • Wet age-related macular degeneration (wet-AMD)
  • Diabetic retinopathy (DR) and macular edema (DME)
  • Retinopathy of prematurity (RoP)
  • All ischemic retinopathies
  • Retinal vessel integrity and perfusivity
  • Corneal neovascularization

Examples:

  • Laser induced choroidal neovascularization (CNV)
  • VEGF induced retinal vasculature permeability
  • Alkali-burn model of corneal neovascularization

Ocular Inflammation Models

Applications:

  • Uveitis (anterior, posterior, both)
  • Bacteria-induced inflammation

Examples:

  • Endotoxin-induced uveitis (Lipopolysaccharide)
  • Experimental autoimmune uveitis
  • Live culture induced inflammation (ocular surface, intracameral, posterior)

Dry Eye Syndrome Models

Applications:

  • Dry eye disease (DED)
  • Keratoconjunctivitis sicca (KCS)

Examples:

  • Scopolamine induced DED in controlled air flow and desiccating environment
  • Benzalkonium chloride induced DED

Glaucoma Models

Applications:

  • Primary open angle glaucoma (POAG)
  • Secondary open angle glaucoma (SOAG)
  • Close angle glaucoma

Examples:

  • AP-2β knockout mouse model for closed angle glaucoma
  • Microbead-induced glaucoma model
  • Steroid-induced chronic ocular hypertension
  • Water loading (hypertonic or isotonic) ocular hypertension

Corneal Wound Healing Models

Applications:

  • Corneal wound healing
  • Neurotrophic keratitis
  • Corneal fibrosis/angiogenesis

Examples:

  • Surface debridement induced by mechanical (incision) or chemical means
  • Corneal fibrosis model
  • Corneal neovascularization model

Ocular Allergy Model

Applications:

  • Seasonal allergic conjunctivitis (SAC)

Examples:

  • Ovalbumin allergic conjunctivitis
  • Histamine-induced allergic conjunctivitis
  • Pollen-induced allergic conjunctivitis

Custom Model Development

Our team of experts at C20/20 have a track record of developing bespoke ophthalmic animal models to suit the needs of industrial and internal partners. We will help you understand what metrics are most relevant to demonstrate efficacy of a novel therapeutic or medical approach in any part of the eye to generate a convincing dataset for future clinical work.

Assessing the distribution and pharmacokinetics of any active molecule is an essential part of any preclinical dataset. Our team uses state of the art liquid chromatography mass spectrometry (LCMS) and a comprehensive ocular dissection protocol to ensure each tissue is collected without cross contamination for industry-leading sensitivity and specificity.

Tissues collected include:

 

  • Conjuctiva

  • Cornea

  • Relevant glandular tissues

  • Aqueous humour

  • Iris/ciliary body

  • Lens

  • Vitreous humour

  • Neural retina

  • Choroid/RPE

  • Other tissues if necessary

Safety/Tolerability

When developing a new chemical entity, formulation or drug delivery system, understanding any potential adverse effects is critical to guide future experiments. We have developed internal methodologies for assessing the safety and tolerability (acute and chronic) of putative therapeutics and materials which can assess their effects with a high degree of granularity.

Our safety and tolerability studies are conducted in rodents, rabbits and other model animals upon request utilizing various routes of administration and include standard toxicology endpoints. All ophthalmic exams are conducted by PhD-trained research scientists with years of experience in this space, lending significant credibility to results obtained.

Endpoints include:

 

  • Cageside and detailed clinical observations

  • Body weight, food consumption

  • Slit lamp ophthalmoscopy with appropriate scoring schema

  • High-resolution imaging

  • Tonometry

  • Electroretinography (ERG)

  • Fluorescein angiography

  • Optical coherence tomography

  • Systemic pharmacokinetics (plasma, serum)

  • Ocular histopathology

  • Immunohistochemistry

Formulation and Delivery

We can provide integrated solutions to develop a wide range of conventional and advanced drug delivery systems for different applications. In ophthalmics, these include in situ gelling systems, contact lenses, microneedles, mucoadhesive eye drops, solid implants, surgical interventions and more.

We also have the necessary equipment and expertise for the fabrication and characterization of nano-formulations.

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Techniques

Our expertise in animal modeling allows us to study a breadth of ocular disease states as well as test our novel ocular biomaterials in vivo. C20/20’s capabilities include:

  • Full histology capabilities including paraffin embedding and cryosectioning with a wide range of staining/immunohistochemistry 

  • Custom genetic model development 

  • Full ophthalmic microsurgery capabilities including subretinal injection, intravitreal and intracameral injection/implantation, dissection, and much more

Equipment

C20/20 has state-of-the-art imaging equipment. Below are some of our specialized ophthalmic imaging instruments:

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  • Heidelberg Spectralis OCT + HRA with posterior, anterior, and wide angle imaging capabilities

  • Phoenix Micron IV fundus camera with full anterior and posterior lens set, OCT module, slit lamp module, and CNV laser module

  • Phoenix Micron X large animal fundus imaging camera

  • Phoenix Ganzfeld ERG 

  • Zeiss slit lamp with video imaging capabilities 

  • Tonolab tonometer 

  • Dissecting microscope 

For a full list of equipment, please visit the Equipment page.